hlif (R&D Systems)

R&D Systems is a verified supplier

R&D Systems manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

R&D Systems hlif
Hlif, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hlif/Recombinant+Human+LIF+Protein/pmc12683510-39-37-40
Average 93 stars, based on 63 article reviews

hlif - by Bioz Stars, 2026-09

93/100 stars

Images

Concentration Assay:

Article Title: Generation of marmoset primordial germ cell–like cells under chemically defined conditions
Article Snippet: .. The cell pellet was resuspended in aRB27 medium, and after adjusting the cell concentration to 5,000 (h and cm) or 10,000 (cj) cells/100 μl/well, the corresponding volume of the cell suspension was added to PGCLC medium (aRB27, 500 ng/ml BMP4 [314-BP; R&D Systems], 10 ng/ml hLIF [SCI], 100 ng/ml SCF [455-MC-010; R&D Systems], 50 ng/ml mEGF [2028-EG-200; R&D Systems], and 10 μM Y-27632 2HCl [S1049; Selleckchem]). .. 100 μl of the cell suspension in PGCLC medium was plated into an ultra-low U-bottom 96-well plate (650979; Greiner or 7007; Costar), and resulting EBs were cultured for 2–6 d.

Suspension:

Centrifugation:

Article Title: Improving in vitro induction efficiency of human primordial germ cell-like cells using N2B27 or NAC-based medium
Article Snippet: The N2B27 medium contained Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (DMEM-F12; Cat. #11330032, Thermo Fisher Scientific) supplemented with N2 (Cat. #17502001, Thermo Fisher Scientific), B27 (Cat. #17504044, Thermo Fisher Scientific), 0.1 mmol/L NEAA, and 0.1 mmol/L 2-mercaptoethanol. hPSCs were treated with TrypLE Express (Cat. #12605028, Gibco) at 37 °C for 3 min, followed by gentle mechanical dissociation using a pipette. .. After centrifugation, the cell pellets were resuspended in GK15 or N2B27 media, supplemented with 200 ng/mL hBMP4 (Cat. #314-BP, R&D Systems), 100 ng/mL hSCF (Cat. #BT-SCF, R&D Systems), 50 ng/mL hEGF (Cat. #236-EG, R&D Systems), 1000 U/mL hLIF (Cat. #7734-LF, R&D Systems), and 10 μmol/L of Y-27632 to induce hPGCLCs. .. Next, 1.5 × 10 6 cells were mixed with 2 mL of culture medium and seeded into one well of the 12-well AggreWell TM 800 culture plate (Cat. #43815, Stem Cell Technologies, Vancouver, BC, Canada) pretreated with an anti-adherence rinsing solution (Cat. #07010, Stem Cell Technologies) following the manufacturer's instructions.

Cell Culture:

Article Title: Compositions and methods for treatment of mitochondrial diseases
Article Snippet: .. NHNP were cultured in neurostem medium that included Neurobasal medium+N2 supplement+10 ng/ml hEGF (Life Technology, Cat. 13247-051; Rockville, Md.) and hLIF (R&D System, Cat. 250-L-050. .. NHNP were cultured in neurostem medium that included Neurobasal medium+N2 supplement+10 ng/ml hEGF (Life Technology, Cat. 13247-051; Rockville, Md.) and hLIF (R&D System, Cat. 250-L-050.

Review